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Nuclear Segmentation on noisy DAPI fluorescent images

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Steven Lewis
Steven Lewis on 26 May 2017
Commented: Steven Lewis on 26 May 2017
I have .tif image stacks for dapi fluorescent microscopy images. I am trying to segment out only the nuclei of the cells, but the images are very noisy and the target signal from the DAPI is diffused into speckled nuclear regions and cytosol. I'm trying to set up an automated thresholding way of doing this with multiple images such as these to segment out only the nuclei but I am extremely new to MATLAB and programming, and everytime I've found discussions about cell segmentation the tools they use do not achieve the same outcome with my images.

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