how to process defocused particle in an image

hi

i am trying to process image of particle , but the issue is that i get defocused particle, so is there any method to adjust the focus for these defocused particles or remove them

i need to do image binarization after solving that issue to measure particle properties and trace theri velocity through successive frame

i attached image for binarized

[darova]

HOw identificate defocused particles?

[khalid Elserfy]

i am still struggling how to identify the defocused particles, but they are clear visually

[darova]

DId you try to change theshold?

[khalid Elserfy]

Yes sure I tried, but I may affect the sizing of the focused object to remove these defocused ones

[darova]

What is the difference between these two? Is one of them defocused?

[khalid Elserfy]

Yes, the faded one is defocused

Answer 1

[darova]

I have an idea:

• Binarize image two times with different threshold
• Separate each blob with bwlabel
• Compare areas of intersected blobs. If A1/A2 < value then delete blob

I = imread('123.png');
I1 = im2bw(I,graythresh(I)-0.3);
I2 = im2bw(I,graythresh(I)+0.0);
subplot(121)
imshow(I)

II = cat(3,I1*0,I2,I1);
subplot(122)
imshow(II)