How to get velocimetry from a single particle image?

Hello everyone. I am a graduate student studying image processing.

I want to velocimetry calculated from a single particle image for the reason that the PIV equipment has failed.

My basic idea is as follows.

1. original image -> rgb2grey -> threshold
2. Calculate the velocity using the 'trajectory length of particle' & 'camera exposure time'

And let me show you example image

You can see the particle trajectory. In my opinion, only the brightest of the many particles will be available for calculation.

And I plan to measure the length per pixel.

So, what I'd like to ask you is can i calculate the velocity field in this idea and, if possible, what code i need.

I look forward to hearing from everyone.

Thank you so much.

[KALYAN ACHARJYA]

"In my opinion, only the brightest of the many particles will be available for calculation"

More brighter pixel avalible, then it can be more velocity?? Is this ?

[JCH]

Thank you for your comment.

I think... If I apply threshold processing, most of the particles will be treated black.

Therefore, I think the particles used for image processing are the brightest things.

The brightness and particles velocity are independent of each other.

Thank you.