measurePSF

Measure a point spread function and other parameters on a microscope
511 Descargas
Actualizado 26 feb 2025

This function measures the full-width-half-max of a microscope point spread function (PSF) along both x and y at the z-plane with the brightest intensity, and also along z/x and z/y. The user simply needs to provide a z-stack containing a PSF (e.g. by imaging a sub-micron fluorescent bead) and also the number of microns per pixel along x/y and z. If using ScanImage (2-photon scanning software) the number of microns per pixel are automatically extracted from the TIFF header.

This submission also contains code and information to measure the number of microns per pixel using an EM grid. If using ScanImage, there is a button in the GUI to apply the measured calibration to ScanImage.

Citar como

Rob Campbell (2025). measurePSF (https://github.com/SWC-Advanced-Microscopy/multiphoton-qc), GitHub. Recuperado .

Compatibilidad con la versión de MATLAB
Se creó con R2019a
Compatible con cualquier versión
Compatibilidad con las plataformas
Windows macOS Linux
Categorías
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Versión Publicado Notas de la versión
2.0.0.0

Update what the submission does.

1.0.0.0

Para consultar o notificar algún problema sobre este complemento de GitHub, visite el repositorio de GitHub.
Para consultar o notificar algún problema sobre este complemento de GitHub, visite el repositorio de GitHub.