I would like to binarize and perform image analysis on this image. such as size and equivalent diameter and count of cells.

Answer 1

[Mandar]

As a potential workaround, you may think of applying segmentation technique such as Watershed segmentation to get the boundaries around different object in the image. The binary segmentation mask can be useful to get the distinct features such as Area, Major and Minor axis lengths, eccentricity etc. using “regionprops” function.

Refer the following MATLAB documentation link to know more about watershed segmentation which demonstrate the applicability of watershed segmentation with example.

Link: https://in.mathworks.com/help/images/marker-controlled-watershed-segmentation.html