Can anyone tell me how to implement and determine a slope for a curve ?

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I´m not experience in matlab, can anyone tell me in this plot how to implement in code or calculate the slope of the blue curve or red curve ? To give me expression of slope?
For example i would like to know the slope of the blue curve between [25;75] (A) ?

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Ricardo Boza Villar
Ricardo Boza Villar el 27 de Feb. de 2016
Maybe this can help you:

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