Matlab Cell Counting from Image w/ Background Light Pollution
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I want to count the number of cells in an image. It's been asked before, I know that. But in the lab that I'm in for my summer internship, sometimes the background becomes polluted with light (ideally it's suppose to be in a dark room but it cannot always be guaranteed).
As a result, here are 3 image examples that I am dealing with:
I had to convert them from a TIF to JPEG so the quality might be slightly reduced
I think us humans can clearly see that there are cells. Each green circle is a nucleus, but it is virtually indistinguishable from the background green pollution.
I've tried all different cell counting methods, including the first 3 pages of Google. Some of the ones that are so close to helping me include: example 1, example 2 and many more
However, I don't know enough about image analysis to determine how to alter those codes to fit my problem.
Any suggestions on identifying the nuclei (those visible to the naked eye) would be incredible. I know it gets hard to see some of them. That is because they turn 'green' when they die. and get more green for a few hours after death. Different cells are just at different walks of life
Posted on stackoverflow and got no answer. Really hoping I can get this solved before work on Monday! I've been stumped for days. Any help would be so much appreciated!
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