i have one doubt please clarify me if we do thresholding for segmenting an area in image then will total counts reduce or decrease

this is the image where i need to take individual frames and apply segmentation to every frame will total counts reduce

Answer 1

[Image Analyst]

Just do simple indexing to extract them.

[rows, columns, numberOfColorChannels] = size(grayImage);
% I'm going to assume numberOfColorChannels is 1 and your images are gray scale not color.
tileRows = floor(rows/8);
tileColumns = floor(columns/9);
r = 1 : tileRows : rows
c = 1 : tileColumns : columns
for row = 1 : length(r)-1
    for col = 1 : length(c)-1
        row1 = r(row);
        row2 = r(row+1);
        col1 = c(col);
        col2 = c(col+1);
        thisImage = grayImage(row1:row2, col1:col2);
        % Now use it, save it, display it or whatever you want.
    end
end

[andhavarapu lokesh]

i want to know if we do thresholding to an image will the total counts reduce or will remain same as before

[Image Analyst]

OK, now you do.

Why don't you just try it and see? If each of those images is thresholded at a different, or even the same, threshold there is no telling the number of blobs. As you apply thresholds to different images you can get different number of blobs in each image, and you can't say that that number will monotonically increase or decrease as you go from one image to the next.

[andhavarapu lokesh]

hi thank you for reply but i want to know if we do thresholding will be there any decrease in counts

[andhavarapu lokesh]

hello can you give me the code of thresholding without gui

[Image Analyst]

Try this:

binaryImage = grayImage > someThresholdValue;

[andhavarapu lokesh]

iam not familiar with matlab please can you tell me i need not require the gui ijust need only original image and thresholded image how should i modify so that i can use the final binary image in to another code please help me

[Image Analyst]

I'm puzzled as to why my line of code above giving you the binary image is not what you want. If you want to modify that image, go ahead. I have no idea how you want to modify it.

If you want to use the final binary image in another program, then either write it out to a file with save() or imwrite() or pass it in via the input argument list of the other program.

Try looking at these:

http://www.mathworks.com/matlabcentral/fileexchange/24224-magic-matlab-generic-imaging-component

http://www.mathworks.com/matlabcentral/fileexchange/25157-image-segmentation-tutorial