center of gravity calculation from a dfiffraction limited spot

I will like to calculate the center of gravity from arrays of spots from a png file. How can I go about it?

[darova]

Did you try regionprops? Please attach the image

[Gift]

Thank you for your response. No, I did not use regionprops. Kindly find attached the image. If possible,I will also like to get the deviation of the spots.

Answer 1

[Bhomik Kankaria]

Hi,

Center of gravity can be calculated using 'regionprops' function from Image Processing Toolbox. You can use centroid as property name to calculate center of gravity in a image.

To know more about the function regionprops, refer to the link below -

https://www.mathworks.com/help/images/ref/regionprops.html

[Gift]

Thank you for yoor rsponse. Please I am new to programming. can you kindly write a code on how it can be done. i.e the COG calculation and the lateral resolution

Answer 2

[darova]

• Try binarize or im2bw to convert image to 0-1 (binary). Experiment with grayscale parameter (see help)
• use regionprops