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How can calculate the average diamter of below image using matlab?

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Amir Torabi
Amir Torabi el 21 de En. de 2020
Editada: Image Analyst el 4 de Mayo de 2021
Hello,
I'd like to calculate the average diamtere of below microstructure using matlab.
Is it possible to calculate it or not?
The actual size of below image is 256um*256um.
ss.jpg

Respuestas (2)

Jeff E
Jeff E el 21 de En. de 2020
I'd start by looking at the regionprops function. Particularly, the "EquivDiameter", "MaxFeretProperties", and "MinFeretProperties" properties. Anything other measurement of diameter of an irregular object will likely be much more difficult to implement, unless you can find something on the Mathworks FileExchange.

Image Analyst
Image Analyst el 22 de En. de 2020
Editada: Image Analyst el 4 de Mayo de 2021
Get a binary image by thresholding the gray scale image. Then clean up a bit and call regionprops(). Assuming the cell walls are darker than the cells:
% Binarize the image by intensity thresholding.
binaryImage = grayImage > 128; % Or whatever. Adjust number as needed or use imbinarize().
% Fill holes, in case there are any.
binaryImage = imfill(binaryImage, 'holes');
% Only keep blobs bigger than 100 pixels in area.
binaryImage = bwareaopen(binaryImage, 100);
% Remove blobs touching border since you can't get reliable diameter if the whole blob is not there.
binaryImage = imclearborder(binaryImage);
% Measure equivalent circular diameters - diameter as if the blob were a perfect circle.
props = regionprops(binaryImage, 'EquivDiameter') % This is a structure.
% Extract Area fields into a double vector.
allDiameters = [props.EquivDiameter]
% Get the average
meanECD = mean(allDiameters)
  4 comentarios
Cristian Mennella
Cristian Mennella el 3 de Mayo de 2021
Editada: Cristian Mennella el 3 de Mayo de 2021
Sorry, instead of props.Area is props.EquivDiameter?or no?
AllDiameters=[props.EquivDiameter]
Image Analyst
Image Analyst el 4 de Mayo de 2021
Yes, you're right. My mistake. I'll correct it.

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